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KMID : 0357419950250020163
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Korean journal of Virology
1995 Volume.25 No. 2 p.163 ~ p.172
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Characterization of Bovine Papillomavirus (BPV) Isolated from Cattle Papilloma
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신원
박남용/임종석/김재화/이인애/박병철/박순희
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Abstract
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The papillomaviruses (PV) produce self-limiting neoplastic diseases in their natural hosts. However, in some cases, they progress into malignancies. The bovine papillomavirus (BPV) is the most oncogenic of the papilloma viruses. BPV 1 is a
causative
agent for bovine fibropapilloma and BPV 4 for bovine alimentary canal cancer. BPV has also served as a prototype for studies on the transformation and molecular biology of the PV. In vitro transformation assay that permitted the analysis of the
viral
functions involved in the induction of cellular proliferation has been developed for the BPV 1. The development of recombinant protein vaccines using bacterial and viral genes by recombinant DNA technology is one of new advanced approaches in
vaccine
production field. We are interested in developing diagnostic kits and vaccines for animal infectious disease agents including bovine papillomaviruses. BPV virion particles and genes are necessary for developing diagnostic technology and vaccines.
Especially, BPV in vitro transformation assay that can be developed using BPV particles is necessary for the verification of the neutralizing efficacy of the vaccines. Therefore in this study we isolated BPV from its natural infection sites on
cattle.
Papilloma tissue surgically removed from cattle skin surface was examined and the presence of hyperplasia induced by BPV infection was confirmed histologically. The sample treated to isolate virus particles was analyzed on CsCi linear gradient
solution
formed by ultracentrifugation. A portion of sample having approximately 1.34 g/ml density matched for the bovine virus partucles density was collected and examined for the presence of BPV capsid antigens by Western blot assay. A 58 KDa band of
expected
size for BPV L1 major capsid protein was identified. thus the isolation of BPV from cattle papilloma was confirmed.
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